Biological preparation for the production of intestinal peristalsis.



UNITED STATES PATENT OFFICE.-

GEORG ZUELZER, 0F BERLIN-CHARLOTTENBURG, GERMANY, ASSIGNOR TO CHEMISCHEFABRIK AUF ACTIEN (VORM. E. SCHERING), OF BERLIN, GERMANY.

BIOLOGICAL PREPARATION FOR THE PRODUCTION OF IIVTESTINAL PERISTALSIS.

No Drawing.

' and metabolic processes. Thus pancreas ferment or rather hormone wasdiscovered as the agent for normal glycolysis and the pro- Y duction ofsuch ferment is described in my application for patent, Serial No.431,226, filed Ma 6, 1908.

Accor ing to the present invention it is possible to produce anotherbiological phenomenon, viz: normal intestinal peristalsis, by means of ahormone or ferment-like substance derived from a suitable digestiveorgan containing this hormone, such as the spleen, the stomach, or theintestine. Hormones differ from ferments as now understood, in thatferments are materially changed ,or rendered incapable of action byboiling, while hormones are not so affected. If such an organ be removedfrom an animal during the process of digestion, the mucous membraneseparated from the muscular tissue and the mucous membrane or even theentire triturated organ treated in a manner suitable for injectionpurposes, it is possible to produce in other animals by injection of theextract a regular paristalsis starting from the duodenum and extendingto the rectum.

The ferment may be produced as follows: For the preparation of thishormone it is desirable to employ the spleen of an animal and the latteris preferably killed during the process of digestlon. The spleen is cutinto small pieces or minced and extracted by means of a physiologicalsolution of com mon salt (containing 8 grams of salt per liter), or adilute solution of muriatic acid (0.4%). The extract is then boiled, thealbuminous precipitate removed by filtration, the filtrate isneutralized, preferably by meansof sodium carbonate, and asepticallyfiltered. If physiological salt solution is employed the extract ispreferably acidulated with dilute muriatic acid. Instead ofSpecification of Letters Patent.

Patented Aug. 6, 1912.

Application filed October 22, 1909. Serial No. 524,008.

extracting the minced spleen by a salt solution or dilute mnriatic acid,as described, the liquid can be extracted by pressure and the juiceresulting from the pressing operation and ,acidulated with dilutemuriatic acid can be treated as described. Instead of the spleen, thestomach or the intestine can be employed by treating these parts ortheir mucous membranes as described, but these parts are less desirable.If the stomach is used the pepsin contained in the liquid is destroyedby the boiling. The filtrate so produced contains the desired hormoneand is preferably used directly as a curative agent by injecting itintothe muscles or veins. The filtrate is preferably concentrated to such anextent that the injection of one cubic centimeter produces in a rabbit adecided peristalsis. The dose for an adult is about 15-20 cubiccentimeters of such a solution, administered by intravenous orintra-muscular injection.

The solution can be evaporated to dryness, preferably in vacuo at 3540C; The dry preparation is a brownish, hygroscopic powder, which isreadily soluble in water, a soda solution or a dilute alkali solution,but is insoluble in alcohol. The alkaline solution gives the biuretreact-ion. The Watery solution can be boiled without changing theefliciency of the preparation.

I claim as my mvention:

1. As a new article of manufacture, the herein described biological prearation containing the hormone which pro uces the normal intestinalperistalsis and which is derived from the digestive'organs of animals,the same bein nearly free from albuminous matter, free fi om tannin,soluble in water, and promotin peristalsis when introduced into theanima organism by intravenous or intramuscular injection, substantiallyas set forth.

2. As a new article of manufacture, the herein described biologicalreparation suitable for the treatment 0 defective peristalsis, whichpreparation, when in a dry state, is a brownish hygroscopic powdersoluble in water, soda solution and dilute alkalis, insoluble inalcohol, and'which, when introduced into the organism, for instance, byintravenous or intramuscular injection, promotes peristalsis,substantially as set forth.

3. The process of manufacturing the herein described biologicalpreparation which consists in producing from suitable digestive organsof an animal a neutral liquid which contains the hormone which producesthe normal intestinal peristalsis, boiling the liquid, removing theprecipitate and filtering such neutral liquid asepticall substantiallyas set forth.

4. The process of manufacturing the here in described biologicalpreparation which consists in mincing a suitable digestive organ of ananimal, extractin therefrom the hormone Which produces t e normalintestinal peristalsis by a Watery extractive solution, acidulating andboiling the extract, separating the precipitate from the liquid byfiltration, neutralizing the filtrate, and filtering aseptically,substantially as set forth.

In testimony whereof I have hereunto set my signature in the presence oftwo subscribing witnesses.

GEOR-G ZUELZER.

Witnesses HENRY HAsPER, WOLDEMAR HAUPT.

